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Additional calculations including the average molecular weight and the degree of ethoxylation for the reference AES sample showed minimum differences (relative error 0.999) was also observed. The individual limit of detection for the target sulphate ethoxymers was determined to be in the low ppm range. Further validated distribution profiles for a large number of AES samples demonstrated the applicability of the optimized HPLC-CAD methodology to routine surfactant screenings. Therefore, the hereby developed methodology provid