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We use the Pt-based colorimetric readout of this assay towards the development of a novel monoclonal antibody (mAb) sandwich pair for the recognition of an anthrax protective antigen (PA83). The identified antibody pair detects PA83 down seriously to 1 nM in phosphate-buffered saline and 5 nM in human serum, that are physiologically appropriate levels. Decreasing gold in the place of platinum onto the mAb-AuNP sandwich enables scanometric recognition of su