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DHEA-S levels were significantly lower among females from opposite-sex twins compared with females from same-sex twins. Our preliminary data do not support the hypothesis that femalesexposed to male co-twins are exposed to higher levels of androgens in utero compared with females exposed to female co-twins. Further studies are needed to explain the reported behavioural and reproductive differences among opposite-sex twins. Our preliminary data do not support the hypothesis that females exposed to male co-twins are exposed to higher leve