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The detection limit of the developed RT-RAA-LFD assay was 10 copies/μL with high specificity for DENV. Compared with commercial reverse transcription quantitative PCR assay, the kappa value of RT-RAA-LFD in 247 clinical samples was 0.957. CONCLUSIONS In this study, a rapid and visual point-of-care test based on RT-RAA and LFD assay was developed and suitable for reliable detection of DENV in low-resource settings with limited laboratory capabilities and optimal storage conditions. BACKGROUND A pneumonia associated with the coronavirus