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Use of high-resolution mass spectrometry (HRMS) including a MS calibration method has enabled simultaneous identification and quantification of knowns/unknowns. This has expanded our knowledge about the existing sample relevant chemical space in a way beyond reconciliation with a quantification task. This is largely due to fact that reference standards are not always available to achieve quantitative analysis. In this scenario, a semi-quantitative approach can fill the gap and provide a rough estimation of concentration. This research a
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