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In summary, we did not confirm our theory that milking cows 2 times a day compared to 3 times in early lactation would lower fat mobilization and lower maximum yield. We performed, however, find an improved milk yield persistency, which partially offset a numerical reduction in maximum yield, and hence there clearly was no considerable effect of reducing early lactation milking frequency on complete lactation (305 DIM) milk yield.within the basic populace, frustration is associated with later depression.