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9 g GAE/kg saffron tepals) and total anthocyanins (2.0 g CGE/kg saffron tepals), reaching 45% and 38% higher values, respectively, as compared to the control sample (without enzymatic treatment). The experimental data regarding optimisation of the extraction conditions were fitted to second-degree regression models (R2 = 0.85-0.98). The newly developed process may be applied as an environmentally friendly alternative to conventional organic solvent extraction, thus supporting valorisation of the saffron industry by-product. The polyph