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The thermodynamic study demonstrated that the adsorption was spontaneous and exothermic. Depending the preparation process, the contribution of each mechanism in the adsorption process was dissimilar. The overall adsorption mechanism was regarded as pore filling, π-π interaction, hydrogen bonding formations, n-π interaction, van der Waals force, and electrostatic attraction. Two methods used to identify the important role of π-π interaction were proposed herein. The possible desorption and reuse of laden-biochars were investigated by