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Previous research reports have demonstrated that traditional slow-freezing cryopreservation processes, yet not storage time, can modify the gene expression profiles of frozen oocytes. Whether vitrification processes and also the related frozen storage durations have impacts regarding the transcriptomes of human metaphase II oocytes stay unknown. Four females (30-32 yrs old) that has undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrif