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Mice emit ultrasonic vocalizations (USVs) that communicate socially relevant information. To detect and classify these USVs, here we describe VocalMat. VocalMat is a software that uses image-processing and differential geometry approaches to detect USVs in audio files, eliminating the need for user-defined parameters. VocalMat also uses computational vision and machine learning methods to classify USVs into distinct categories. In a data set of 4000 USVs emitted by mice, VocalMat detected over 98% of manually labeled USVs and accur