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The α-arbutin yield of 60.3% was accomplished from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. TIPS • A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized. • SaAS has the highest certain enzyme task among all known amylosucrase. • SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities.Brown algae are thought encouraging crops for the production of lasting biofuels. Nonetheless, the commercial application is limited by not enoug