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In this study, first, graphitic carbon nitride was treated with hydrogen peroxide (abbreviated as H2O2-g-C3N4), then was used as a new hydrophilic nanomaterial in the fabrication of polyethersulfone (PES) mixed matrix membrane (MMM) for improving flux, protein and dye separation efficiency and antifouling properties. The H2O2-g-C3N4 nanosheet was inserted into the doping solution to fabricate PES/H2O2-g-C3N4 nanocomposite membrane with the non-solvent induced phase inversion procedure. The results of the SEM and AFM images and also poro