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Here, we used ChIP-seq on synchronized cells at different phases after mitosis to create genome-wide maps of histone alterations. Combined with EU-RNA-seq and Hi-C analyses, we found that during prometaphase, promoters, enhancers, and insulators retain H3K4me3 and H3K4me1, while losing H3K27ac. Enhancers globally maintaining mitotic H3K4me1 or locally retaining mitotic H3K27ac are associated with mobile type-specific genetics and their transcription aspects for quick transcriptional activation. As cell