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Recombinant CarE001A and CarE001H expressed in the Escherichia coli exhibited large enzymatic task toward α-naphthyl acetate. Inhibition assays showed that organophosphates had strong inhibition on CarEs task compared to pyrethroids. Metabolism assays indicated that CarE001A and CarE001H were able to metabolize β-cypermethrin and λ-cyhalothrin. Homology modeling and molecular docking analyses demonstrated that β-cypermethrin could fit well into the energetic pocket of both carboxyle