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The overall prevalence of GBS carriage was 10.1% (130/1,281, 95% CI 8.5-12.1%) according to the standard reference method. Compared with the standard reference method, the LAMP had excellent performance of sensitivity (100%, 95%CI 83.4-100%), specificity (94%, 95%CI 88.1-97.1%), and Yoden index (0.94; as well as the blood agar plate with sensitivity (81.5%, 95%CI 61.3-93.0%), specificity (100%, 95%CI 98.3-100.0%), and Yoden index (0.815). The other four methods were not sufficient to reach the threshold in terms of sensitivity or speci