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The Michaelis constant (Km) was increased and the optimum temperature of lipase was widened observably after crosslinking with PEI on PEI@PMMA magnetic microspheres. Hydrogen gas therapy has been recognized as the promising application merit. However, the underlying mechanism on the biological effects remains far from being understood. In this work, pepsin used as a research model, the effects of hydrogen-rich water on the protein activities and structural properties were investigated by enzymatic assay, atomic force microscopy-based p
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