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A key challenge in the process of virus amplification is the need for a simple and convenient method for measuring virus titers. Real-time unlabeled cell analysis (RTCA) was used to establish a standard curve of correlation between half-cell index time (CIT ) and virus titer. At the same time, the virus titer from tunable resistance pulse detection (TRPS) technology was compared with the traditional median tissue culture infectious dose (TCID ) method to evaluate the feasibility and application value of the RTCA technique and TRPS techn