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998 and 0.960, respectively); TMB reproducibility was 0.996 (concordance correlation coefficient). Limit of detection for all variant classes was less then 20% tumor content. Together, we demonstrate that multiplex PCR/semiconductor sequencing-based tumor tissue CGP is feasible using optimized bioinformatic approaches described herein.Discovery of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has greatly improved our gene editing technology. Their applications in the area of diagnostic innovation are ga