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grain matrix used was dry distillers grains with solubles (referred to as DG, henceforth), the most prevalent type of distillers coproduct in the marketplace. Subsequently, the method was approved for a multi-laboratory collaborative validation (MLV) to adequately verify it to be robust and reliable for FDA's compliance and regulatory actions. The objective of this study was to carry out a collaborative MLV of the LC-MS/MS analytical method developed in FDA/CVM laboratory (4) to determine residues of erythromycin, penicillin G, virginiamyc