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Introduction Hand hygiene is crucial to prevent cross infection. Healthcare students are in a prime position to learn hand hygiene skills. The aim of this study was to analyze hand hygiene behavioral intentions of healthcare students before and after contact with the patient and to compare the knowledge of and attitude towards hand hygiene between medical and nursing students. Methods In a descriptive survey research design, convenience selection of a sample of medical students (n=657) and nursing students (n=303) was done from modules t