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For examples co-incubated with an income biofilm, a luciferase assay had been performed to be able to assess its viability. Surfaces were impressed before and after each storage problem and replicas examined in a scanning electron microscope. Utilizing imagher gap portion in the margins, verifying the unfavorable effectation of cariogenic bacteria on margin degradation. The parameters defined for such synergy can help understand the multi-factorial part of marginal d