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By quantitatively blocking surface thiols with a thiol binding ligand (qBBr), we show that surface thiols don't have any significant influence on Hg(II) methylation, regardless of Cys addition. The outcome additionally identify a significant level of cell-associated Hg-S3/S4 species, as studied by large energy-resolution X-ray absorption near edge structure (HR-XANES) spectroscopy, under problems of high MeHg production (with Cys addition). On the other hand, Hg