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Human dermal papilla (DP) cells and melanocytes (hMel) are central players in hair growth and pigmentation, respectively. In hair follicles (HFs), oxygen (O ) levels average 5%, being coupled with the production of reactive oxygen species (ROS), necessary to promote hair growth. DP cell and hMel proliferation and phenotype were studied under physiological (5%O , physoxia) or atmospheric (21%O , normoxia) oxygen levels. hMel-DP cells interactions were studied in indirect co-culture or by directly co-culturing hMel with DP spheroids, to tes