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9993-0.9996), sensitive (limit of detection, LOD ≤ 0.8 ng mL-1), accurate (89.3-114.2%), and precise (relative standard deviation, RSD ≤ 6.23%). These results indicated that the proposed method is qualified in bioanalytical method validation and meets the requirements for detecting illegally used antiviral drugs in yellow catfish. The demonstrated G/KCC-1 based PT-SPE UPLC-MS/MS method is a potential analytical method in food and drug administration.Spinal cord injury (SCI) is a leading cause of disability and death worldwide. Reactive a