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Copyright © 2020 Author(s).A significant barrier into the clinical utilization of microfluidically created water-in-oil droplets is the cumbersome washing actions expected to remove the non-biocompatible natural oil stage through the droplets. In this report, we report an on-chip magnetic water-in-water droplet generation and manipulation system making use of a biocompatible aqueous two-phase system of a polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer (PEG-PPG-P