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To generate data for design assessment three challenge tests were performed with mould-ripened cheeses generated by using milk inoculated with L. monocytogenes. Growth of L. monocytogenes and lactic acid bacteria (LA in the skin and in the core of cheeses had been quantified along with alterations in item traits in the long run (temperature, pH, NaCl/aw, lactic- and acetic acid concentrations). The performance of nine available L. monocytogenes gr