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To demonstrate the power of this automated image analysis, we used a commercial maturation medium intended to promote the maturation status of hiPSC-CM, and compare the parameters with the ones obtained with standard culture medium, and with freshly dissociated mouse cardiomyocytes. Supplementary data are available at Bioinformatics online. Supplementary data are available at Bioinformatics online.Metachronal motion is used across a wide range of organisms for a diverse set of functions. However, despite its ubiquity, analysis of this be