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In this context, we created a tool that can rotate immediately inside the confines of an enclosed dark package, and utilizing this, we can record [Ca2+ ]cyt dynamics immediately after flowers have been rotated to cause technical stimulation. This device can facilitate the analysis of perception and early signal transduction in reaction to technical stimulation on a sizable scale centered on [Ca2+ ]cyt responses. © 2020 Wiley Periodicals LLC. Basic Protocol 1
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