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The constructed chamber for multi-wavelength imaging is an inexpensive alternative (ca. 20 Euros) to the commercially available densitometers. The discussed approaches for image acquisition and chemometric data processing support a more reliable and objective analysis of TLC multi-wavelength data.Dimethyl sulfide (DMS) is the major biogenic volatile sulfur compound in surface seawater. Good quality DMS data with high temporal and spatial resolution are desirable for understanding reduced sulfur biogeochemistry. Here we present a fully a