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A maximum selectivity of 65% was obtained for the mixed amino acids via one-step separation. These PAAILMs have the advantages of low operating pressure, high water flux, good antibacterial and antifouling properties, and excellent reusability, thereby indicating their potential for industrial application in the separation of l-phenylalanine and l-aspartic acid.Understanding the stability evolution of the silver nanoparticles (Ag NPs) in colloid has great benefits for its controllable preparation, storage and application. Herein, uncapped A