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The clear presence of Rickettsia ended up being determined by PCR and sequencing. Results 10/39 work vials amplified fragments regarding the gltA, htrA and ompB genetics. On 7/10 from Rh. sanguineus s.l. collected from dogs plus in 3/10 of A. mixtum obtained from horse and human. Sequencing indicated R. typhi in Rh. sanguineus and A. mixtum with 100% homology (LS992663.1) for an area for the htrA gene and 99per cent (LS992663.1) with the areas of the gltA and OmpB genetics. The minimal
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