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d in further studies. In conclusion, it is recommended for future research to use cut instead of plucked feathers for corticosterone measurement.Live sheep export has become a public concern. This study aimed to test a non-contact biometric system based on artificial intelligence to assess heat stress of sheep to be potentially used as automated animal welfare assessment in farms and while in transport. Skin temperature (°C) from head features were extracted from infrared thermal videos (IRTV) using automated tracking algorithms. Two para