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We expect that this approach can be developed as a next generation biochip for biomedical research and industries.Polymer optical fibers (POFs) were used for preparing side-emitting polymer optical fibers (SPOFs), which were processed with acetone and n-hexane combined in selected proportions by a solvent treatment method. The effects of the volume ratio of acetone to n-hexane and treatment time on response variable factors were investigated. The center composite design (CCD) based response surface methodology (RSM), a quadratic model,