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Rhinoncomimus latipes Korotyaev is a specialist biocontrol agent of mile-a-minute weed, Persicaria perfoliata (L.) H. Gross (Caryophyllales Polygonaceae). Currently, R. latipes is released by hand where the presence of the weed is readily detected. However, the hand-release method is not applicable to weed patches spread in hard-to-access areas. This study was conducted to develop a spatially targeted biocontrol strategy by using an unmanned aircraft system (UAS, a.k.a. drone) for the detection of P. perfoliata and aerial release of R.