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The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis (MLVA) of of Beijing in 2016 in pediatric patients. Real-time quantitative polymerase chain reaction (PCR) was used to identify , and MLVA was performed. The domain V of the 23S rRNA was sequenced to detect macrolide-resistant point mutations. We also investigated the activities of antibiotics against isolates . The PCR detection rate of in children in Beijing was 40%, and the macrolide res