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Segments from other species have been introduced into the H16N3 avian influenza virus, which may alter its pathogenicity and host tropism, potentially posing a threat to animal and human health in the future. Consequently, it is necessary to increase monitoring of the emergence and spread of avian influenza subtype H16N3 in wild birds. © 2020 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.AIM The primary objective of this study was to assess the ultrasonographic signs of subclinical enthesitis in