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The association between the pks-1 gene expression and chaetoglobosin A production was estimated. The Pks-1 gene was expressed by all C. globosum isolates except one isolate, C1, which is another species of Chaetomium. In addition, all C. globosum isolates produced chaetoglobosin A with different concentrations and did not express the same levels of pks-1. This finding may be a result of the solvent type used in the extraction. © 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.Acute leukemias of ambiguous lineage (ALAL) comprise acut