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Furthermore, PCR analysis revealed that the c880t mutation into the fadD32 gene first appeared in the first-step mutant, A2 (MIC 1 mg/L). Complementation associated with the wild-type M61 utilizing the pMV261 plasmid carrying the mutant fadD32 gene caused the previously painful and sensitive M61 to develop a lower susceptibility to linezolid (MIC 1 mg/L). The results for this study uncovered hitherto undescribed systems of linezolid opposition in M. abscessus that could be useful for the intro