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An incubation experiment was conducted to investigate whether different biochar could enhance the biodegradation of sulfamethoxazole (SMX) and chloramphenicol (CAP). During incubation in nutrient medium solution, the degradation efficiencies of SMX by P. stutzeri and S. putrefaciens obtained 61.79% and 68.67% respectively, while CAP was 85.75% and 85.70%. The biodegradation efficiencies of SMX and CAP increased for P. stutzeri cultured with biochar and increased for S. putrefaciens cultured with high-concentration biochar (500, 1,000, 2,