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The higher inhibition zone diameters for the CT-TG/SiO2@Ag (1/1) nanocomposite film against both bacteria were observed in the presence of aqueous solution of acetic acid 1% v/v (11 and 13 mm) and deionized water (18 and 20 mm), respectively. The characteristics of single domain and ease of gene manipulation of the single domain antibody (sdAb) make it suitable for affinity maturation in vitro. Since the affinity of antibodies can influence the immunoassays' sensitivity, a nanobody (Nb), the anti-ochratoxin A sdAb (AOA-sdAb), was herei