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cultures were recovered from symptomatic leaves of all inoculated plants and verified as similar in morphology to the original C. cassiicola isolates, thus fulfilling Koch's postulates. Based on morphology, pathogenicity, and sequence data results, this is the first report of C. cassiicola causing Target spot on soybean in the Eastern Plains of Colombia and expands our knowledge of this disease. Target spot poses a threat to the expanding soybean crops in this country, as the range of yield losses due to this disease, in South Am