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These results strongly suggest that the level of endothelin-1 plays an important role in the pathogenesis of immune-mediated CNS demyelinating diseases by promoting immune responses. The aim of this study is to investigate the associations between post-stroke cognitive impairment (PSCI) severity and reactive astrogliosis (RA) extent on normalized F-THK-5351 positron-emission tomography (PET) imaging in amyloid-negative patients with first-ever stroke. We prospectively enrolled 63 amyloid-negative patients with first-ever stroke. Neuroc