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β-Cyclodextrin can be functionalized by derivation of reactive hydroxyl on the ring due to its special chiral environment and structural characteristics, which can be used to identify or separate a variety of chiral substance. In this manuscript, a series of excellent chiral stationary phases for high-performance liquid chromatography were developed for enantioseparation by using anhydride modified β-cyclodextrin bearing chiral (R/S)-α-phenethylamine or (S)-(+)-2-amino-1-propanol. They were characterized by elemental analysis, Fourier t