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nto more in vivo-like cardiac tissue in the future.The rhizobia isolated from root nodules of horse gram were screened for nickel (Ni) tolerance in vitro. The strain HGR-4 could tolerate 1000 µg g-1 of Ni. It was also observed that horse gram plants associated with HGR-4 have shown Ni stress tolerance in Ni amended soils up to a concentration of 100 µg g-1. In another experiment, the plants associated with HGR-4 have shown higher nodulation, nitrogen level, and leghaemoglobin content at 80 µg g-1 of Ni than control plants without HGR-4