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This is basically the only known non-H5/H7 virus with four fundamental proteins when you look at the HACS. Right here, we investigated the virulence of the virus in chickens after growth of this polybasic CS by substitution of T327R (322PEKRRRR/G329) or T327K (322PEKRRKR/G329) with or without reassortment with HPAIV H5N1 and H7N7. The effect of solitary mutations or reassortment on virus fitness in vitro plus in vivo had been studied. Efficient mobile tradition
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