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Also, we found that AgNOR staining for the HPV 16/18 group was significantly greater than for the HPV negative group. We found that CRP level, cell proliferation and genome instability were increased in HPV positive patients. The AgNOR and micronucleus tests were useful for evaluating cell proliferation and DNA damage.Electrosurgical radio-frequency heating of tissue is widely applied in minimally invasive surgical procedures to dissect tissue with simultaneous coagulation to obtain hemostasis. The tissue effect depends on the