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Affinity ultrafiltration strategy can screen substances from compounds rapidly, but G-quadruplex DNA ligands are tough to dissociate, that is a vital step up main-stream ultrafiltration technique. In this paper, the filtrates after ultrafiltration were based on HPLC-MS in replacement. The peaks with 20% reduced total of MS reaction through the incubation vs control had been regarded as ligand components to G-quadruplex. Two associated with peaks using the relative abundance above 3