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In vitro, selective inhibition of GADPH suppressed the growth and metastasis of Huh-7, Bel7404 and Hep3B hepatocellular carcinoma cell lines. The Warburg effect may play an important role in GI cancers, especially in liver cancer. The Warburg effect may play an important role in GI cancers, especially in liver cancer. The transforming growth factor β regulator 4 (TBRG4) has been proved to be involved in various types of tumor. However, its contribution in human osteosarcoma (OS) is still unclear. In the present study, immunohistochemist