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0-160 μg kg-1. The limits of detection (LOD) and quantification (LOQ) achieved were 4.0 and 8.0 μg kg-1, respectively. This LOQ value is lower than the maximum residue limit established by the Brazilian and European Union legislation. Therefore, the LLE-LTP combined to HPLC-DAD allows the routine analysis of DDT in honey samples and can be widely applied in studies to monitor this pesticide, especially in developing countries, where DDT use is still allowed.Extracellular vesicles (EVs) are nanoscale vesicles with a phospholipid bilayer.