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We also report the very first time research connecting the RTD and the general amino acid control (GAAC) paths, which we show in both S. pombe and S. cerevisiae. In S. pombe trm8Δ mutants, natural GAAC mutations restored heat resistance and tRNA levels, and also the trm8Δ temperature sensitivity had been precisely linked to GAAC activation due to tRNATyr(GUA) decay. Likewise, within the well-studied S. cerevisiae trm8Δ trm4Δ RTD mutant, temperature sensitivity ended up being cl